Direct PCR in Forensic Science- An overview
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چکیده
Direct PCR was first used in the field of microbiology, where it was more commonly known as colony PCR. Since then, many more applications of direct PCR has been described in other fields where it has aided in diagnosis of infectious diseases and in botany. Direct PCR is a technique where amplification is carried out on samples without prior extraction, purification or quantification. In forensic DNA, direct PCR is currently limited to amplifying blood and buccal stained FTA cards using specially designed multiplex kits. These multiplex kits have improved buffer-polymerase systems which are more tolerant to inhibitors present on FTA samples. In 2004, the UK government highlighted the need to continue improving police capabilities in the areas of recovering evidence and rapid analysis of body fluids and other relevant forensic samples. Based on these requests, several key drivers were identified to include the development of faster, cheaper and better forensic science, which is possible with direct PCR. The need for faster, cheaper, better forensic science also becomes more apparent with the increasing number of samples subjected for DNA analysis after the implementation of programmes such as the likes of the DNA Expansion Programme in the UK and the DNA Field Experiment in the US. However, just like any other scientific technique, direct PCR does have its pitfalls which could be overcome with several modifications to the conventional DNA profiling workflow.
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تاریخ انتشار 2015